Video: Isolation and Characterization of RNA-Containing Exosomes
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Video: Isolation and Characterization of RNA-Containing Exosomes
100.0K Views. University of Gothenburg. The overall goal of the following experiment is to determine whether cells and biological fluid of interest release or contain RNA containing exosomes. The first step to isolate exosomes is to remove cells and cell debris. Next, the supernat is then filtered to remove larger particles.The exosomes are ...
100.3K viewsJan 9, 2012
RNA Extraction Methods
RNA Extraction Method (Guanidinium Thiocyanate-Phenol Method) The extraction of RNA is an essential and basic step in any molecular biology experiment such as RT-PCR, cDNA library construction, sequencing, gene expression studies, microarray analysis, etc. There are different types of methods available like CsCl density gradient method, silica column based method but the guanidinium thiocyanate or guanidium isothiocyanate-phenol-chloroform method is the most important and is the common one. Now
7:42
RNA Extraction Method (Guanidinium Thiocyanate-Phenol Method) The extraction of RNA is an essential and basic step in any molecular biology experiment such as RT-PCR, cDNA library construction, sequencing, gene expression studies, microarray analysis, etc. There are different types of methods available like CsCl density gradient method, silica column based method but the guanidinium thiocyanate or guanidium isothiocyanate-phenol-chloroform method is the most important and is the common one. Now
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